26dkou617 10.3205/26dkou617 urn:nbn:de:0183-26dkou6177 Meeting Abstract Zheng Zheng Junwen J

Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Gießen, Deutschland

author Jahed Jahed Vahid V

Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Gießen, Deutschland

author Rastegar Adib Rastegar Adib Fatemeh F

Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Gießen, Deutschland

author Heiss Heiss Christian C

Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Gießen, Deutschland Department of Trauma, Hand and Reconstructive Surgery, University Hospital of Gießen-Marburg, Gießen, Deutschland

author El Khassawna El Khassawna Thaqif T

Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig-University, Gießen, Deutschland

author German Medical Science GMS Publishing House

Düsseldorf

610 20261020 engl This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). M0659 617 Deutsche Gesellschaft für Orthopädie und Unfallchirurgie Deutsche Gesellschaft für Orthopädie und Orthopädische Chirurgie Deutsche Gesellschaft für Unfallchirurgie Berufsverband für Orthopädie und Unfallchirurgie Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2026) Posterpreise experimentell Berlin 20261020 20261023 DKOU26_Abs_2354 TextObjectives and questions: Bone infection (osteomyelitis) is a major complication in orthopaedic surgery and is frequently associated with biofilm formation and antibiotic -resistant strains like methicillin-resistant Staphylococcus aureus (MRSA). While myrrh oil possesses known antibacterial and antioxidant properties, its clinical application is hindered by poor aqueous solubility. This study developed a water-soluble inclusion complex of myrrh oil with hydroxypropyl-β-cyclodextrin (HP-β-CD) and incorporated it into an injectable gelatin methacryloyl (GelMA) hydrogel to create a biocompatible, antibacterial scaffold for localized infection management.Material and methods: Myrrh oil was extracted via rotary vacuum evaporation and complexed with HP-β-CD to enhance solubility. Antibacterial efficacy of the myrrh–CD complex (0–50% w/v) was evaluated using liquid culture and agar diffusion assays against Escherichia coli, S. aureus, and MRSA. Cytocompatibility was assessed via CCK-8 assays using human-derived stem cells. Antioxidant potential was quantified using the DPPH radical-scavenging assay. Finally, the complex was integrated into a photocrosslinkable GelMA hydrogel (3:1:10 weight ratio) to evaluate the biocompatibility of the 3D formulation.Results: The myrrh–CD inclusion complex demonstrated significant antibacterial activity at concentrations ≥10% (w/v), achieving >90% reduction in bacterial growth compared to controls (p < 0.01). Agar diffusion assays yielded clear inhibition zones for S. aureus (10.54 mm ± 0.11 mm) and MRSA (10.11 mm ± 0.24 mm). The complex exhibited dose-dependent antioxidant activity, reaching 50% radical scavenging at a concentration of 10% (w/v), while CD alone showed no measurable activity. Cytocompatibility assays confirmed no detectable cytotoxicity, with stem cell viability remaining >90% after 24 hours of exposure to the complex. Furthermore, the GelMA-based 3D hydrogel preserved high cell viability upon direct contact, confirming the maintenance of biocompatibility within the final scaffold formulation.Discussion and conclusions: Complexation with HP-β-CD successfully solubilizes myrrh oil while preserving its potent antibacterial and antioxidant properties. The resulting myrrh–CD complex shows significant efficacy against resistant strains like MRSA without inducing cytotoxicity in human-derived stem cells. Preliminary incorporation into an injectable GelMA hydrogel maintains high cell viability, supporting its potential as a biocompatible 3D scaffold for the local treatment of bone infections. 0 0 0 0