26rhk030
10.3205/26rhk030
urn:nbn:de:0183-26rhk0304
Meeting Abstract
Distinct age- and therapy-specific single cell-signatures in the intestinal microbiota of juvenile idiopathic arthritis patients
Sempert
Sempert
Toni
T
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
Department for Cytometry, Institute of Biotechnology, Technische Universität Berlin, Berlin, Deutschland
author
Budzinski
Budzinski
Lisa
L
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Lietz
Lietz
Leonie
L
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Maier
Maier
René
R
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Kang
Kang
Gi-ung
Gu
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
von Stuckrad
von Stuckrad
Anne Sae Lim
ASL
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin Berlin, Berlin, Deutschland
German Center for Child and Adolescent Health (DZKJ), partner site Berlin, Berlin, Deutschland
author
Goetzke
Goetzke
Carl Christoph
CC
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
German Center for Child and Adolescent Health (DZKJ), partner site Berlin, Berlin, Deutschland
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin Berlin, Berlin, Deutschland
author
Shah
Shah
Aayushi
A
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Abbas
Abbas
Amro
A
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Lehmann
Lehmann
Katrin
K
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Bartsch
Bartsch
Stefanie
S
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin Berlin, Berlin, Deutschland
author
Necke
Necke
Kathleen
K
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin Berlin, Berlin, Deutschland
author
Hoffmann
Hoffmann
Ute
U
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
author
Mashreghi
Mashreghi
Mir-Farzin
MF
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
German Center for Child and Adolescent Health (DZKJ), partner site Berlin, Berlin, Deutschland
author
Kallinich
Kallinich
Tilmann
T
German Center for Child and Adolescent Health (DZKJ), partner site Berlin, Berlin, Deutschland
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité Campus Virchow, Charité Universitätsmedizin Berlin, Berlin, Deutschland
author
Chang
Chang
Hyun-Dong
HD
German Rheumatology Research Center Berlin (DRFZ) – A Leibniz Institute, Berlin, Deutschland
Department for Cytometry, Institute of Biotechnology, Technische Universität Berlin, Berlin, Deutschland
author
German Medical Science GMS Publishing House
Düsseldorf
610
20260909
engl
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung).
M0656
030
Deutsche Gesellschaft für Rheumatologie
Deutsche Gesellschaft für Orthopädische Rheumatologie
Gesellschaft für Kinder- und Jugendrheumatologie
54. Kongress der Deutschen Gesellschaft für Rheumatologie und Klinische Immunologie (DGRh), 36. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR), 40. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh)
Deutscher Rheumatologiekongress 2026
Experimentelle & Translationale Rheumatologie
Leipzig
20260909
20260912
ET.02
TextIntroduction: Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with chronic inflammatory diseases. In this study, we have characterized the JIA microbiota taxonomically and phenotypically in relation to age dynamics and therapy response to methotrexate (MTX).Methods: We have analyzed the intestinal microbiota of 54 JIA patients and 38 age-matched healthy controls using 16S rRNA sequencing and multi-parameter microbiota flow cytometry (mMFC). With mMFC, we interrogate coating of bacteria with endogenous host immunoglobulins by isotype-specific stainings and the surface expression of specific sugar moieties using sugar-specific plant-derived lectins. The multivariate data are analyzed with machine learning algorithms to define disease- or therapy-specific microbial signatures.Results: We found that age is a major confounder in the microbiota analyses of children. When stratifying patients and healthy controls according to age into three groups, we could show that each age group has a distinct, non-overlapping taxonomic and phenotypic signature (PERMANOVA, all p < 0.05). Specifically, we identified novel bacterial species associated with JIA not seen in the non-stratified comparison between JIA patients and healthy controls. Furthermore, we also identified age group-specific phenotypic signatures differing between JIA patients and healthy controls based on immunoglobulin coating and lectin staining. Using mMFC and based on microbial signatures, we were able to discriminate responders and non-responders to MTX-therapy (PERMANOVA, R2= 0.28, p=9.9*10-5) and also to predict therapy responsiveness (AUC = 0.873, 95 % CI: 0.691 – 1).Conclusion: Our results highlight the dynamic nature of the intestinal microbiota in pediatric patients and show the necessity to account for age in microbiome analyses in children. The mMFC approach shown here, demonstrates that single-cell analysis of the intestinal microbiota from stool samples has the potential to impact not only therapy monitoring but also therapy prediction, a first step towards microbiota-guided personalization of medicine.Disclosures: The authors declare that they have no competing interests.
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